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Figure 5. Sildenafil restores phospho-Smad1/5 and Inhibitor of DNA binding protein 1 (Id1) protein levels in monocrotaline (MCT)-exposed rat lungs. Immunoblots showing expression of bone morphogenetic protein type II receptor (BMPR-II) and phospho-Smad1/5 in control rat lungs (Con) and rats exposed to MCT and treated with saline (MCT) or sildenafil (MCT+Sil) for 2 weeks (A). Accompanying graphs show results of densitometry for BMPR-II (B) and phospho-Smad1/5/8 (C). Real-time <t>qPCR</t> evaluation of Id1 gene expression level in control (Con) and MCT-exposed rats treated with saline (MCT) or sildenafil (MCT+Sil) (D). *P<0.05 compared with control; #P<0.05 compared with MCT. Panel shows confocal microscopy images of a small pulmonary artery from a control rat (Con), and a rat exposed to MCT+saline (MCT) or sildenafil (MCT+Sil) showing the colocalization of α-SMA and phospho-Smad1/5 (E). Scale bar, 20 µm.
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Figure 5. Sildenafil restores phospho-Smad1/5 and Inhibitor of DNA binding protein 1 (Id1) protein levels in monocrotaline (MCT)-exposed rat lungs. Immunoblots showing expression of bone morphogenetic protein type II receptor (BMPR-II) and phospho-Smad1/5 in control rat lungs (Con) and rats exposed to MCT and treated with saline (MCT) or sildenafil (MCT+Sil) for 2 weeks (A). Accompanying graphs show results of densitometry for BMPR-II (B) and phospho-Smad1/5/8 (C). Real-time <t>qPCR</t> evaluation of Id1 gene expression level in control (Con) and MCT-exposed rats treated with saline (MCT) or sildenafil (MCT+Sil) (D). *P<0.05 compared with control; #P<0.05 compared with MCT. Panel shows confocal microscopy images of a small pulmonary artery from a control rat (Con), and a rat exposed to MCT+saline (MCT) or sildenafil (MCT+Sil) showing the colocalization of α-SMA and phospho-Smad1/5 (E). Scale bar, 20 µm.
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Figure 5. Sildenafil restores phospho-Smad1/5 and Inhibitor of DNA binding protein 1 (Id1) protein levels in monocrotaline (MCT)-exposed rat lungs. Immunoblots showing expression of bone morphogenetic protein type II receptor (BMPR-II) and phospho-Smad1/5 in control rat lungs (Con) and rats exposed to MCT and treated with saline (MCT) or sildenafil (MCT+Sil) for 2 weeks (A). Accompanying graphs show results of densitometry for BMPR-II (B) and phospho-Smad1/5/8 (C). Real-time <t>qPCR</t> evaluation of Id1 gene expression level in control (Con) and MCT-exposed rats treated with saline (MCT) or sildenafil (MCT+Sil) (D). *P<0.05 compared with control; #P<0.05 compared with MCT. Panel shows confocal microscopy images of a small pulmonary artery from a control rat (Con), and a rat exposed to MCT+saline (MCT) or sildenafil (MCT+Sil) showing the colocalization of α-SMA and phospho-Smad1/5 (E). Scale bar, 20 µm.
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tiangen biotech co superreal fluorescence quantitative premix reagent
Figure 5. Sildenafil restores phospho-Smad1/5 and Inhibitor of DNA binding protein 1 (Id1) protein levels in monocrotaline (MCT)-exposed rat lungs. Immunoblots showing expression of bone morphogenetic protein type II receptor (BMPR-II) and phospho-Smad1/5 in control rat lungs (Con) and rats exposed to MCT and treated with saline (MCT) or sildenafil (MCT+Sil) for 2 weeks (A). Accompanying graphs show results of densitometry for BMPR-II (B) and phospho-Smad1/5/8 (C). Real-time <t>qPCR</t> evaluation of Id1 gene expression level in control (Con) and MCT-exposed rats treated with saline (MCT) or sildenafil (MCT+Sil) (D). *P<0.05 compared with control; #P<0.05 compared with MCT. Panel shows confocal microscopy images of a small pulmonary artery from a control rat (Con), and a rat exposed to MCT+saline (MCT) or sildenafil (MCT+Sil) showing the colocalization of α-SMA and phospho-Smad1/5 (E). Scale bar, 20 µm.
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Image Search Results


Figure 5. Sildenafil restores phospho-Smad1/5 and Inhibitor of DNA binding protein 1 (Id1) protein levels in monocrotaline (MCT)-exposed rat lungs. Immunoblots showing expression of bone morphogenetic protein type II receptor (BMPR-II) and phospho-Smad1/5 in control rat lungs (Con) and rats exposed to MCT and treated with saline (MCT) or sildenafil (MCT+Sil) for 2 weeks (A). Accompanying graphs show results of densitometry for BMPR-II (B) and phospho-Smad1/5/8 (C). Real-time qPCR evaluation of Id1 gene expression level in control (Con) and MCT-exposed rats treated with saline (MCT) or sildenafil (MCT+Sil) (D). *P<0.05 compared with control; #P<0.05 compared with MCT. Panel shows confocal microscopy images of a small pulmonary artery from a control rat (Con), and a rat exposed to MCT+saline (MCT) or sildenafil (MCT+Sil) showing the colocalization of α-SMA and phospho-Smad1/5 (E). Scale bar, 20 µm.

Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: Sildenafil Potentiates Bone Morphogenetic Protein Signaling in Pulmonary Arterial Smooth Muscle Cells and in Experimental Pulmonary Hypertension

doi: 10.1161/atvbaha.112.300121

Figure Lengend Snippet: Figure 5. Sildenafil restores phospho-Smad1/5 and Inhibitor of DNA binding protein 1 (Id1) protein levels in monocrotaline (MCT)-exposed rat lungs. Immunoblots showing expression of bone morphogenetic protein type II receptor (BMPR-II) and phospho-Smad1/5 in control rat lungs (Con) and rats exposed to MCT and treated with saline (MCT) or sildenafil (MCT+Sil) for 2 weeks (A). Accompanying graphs show results of densitometry for BMPR-II (B) and phospho-Smad1/5/8 (C). Real-time qPCR evaluation of Id1 gene expression level in control (Con) and MCT-exposed rats treated with saline (MCT) or sildenafil (MCT+Sil) (D). *P<0.05 compared with control; #P<0.05 compared with MCT. Panel shows confocal microscopy images of a small pulmonary artery from a control rat (Con), and a rat exposed to MCT+saline (MCT) or sildenafil (MCT+Sil) showing the colocalization of α-SMA and phospho-Smad1/5 (E). Scale bar, 20 µm.

Article Snippet: DNA samples were analyzed by real-time PCR amplification using KAPA SYBR FAST qPCR reagents (Kapabiosystems).

Techniques: Binding Assay, Western Blot, Expressing, Control, Saline, Gene Expression, Confocal Microscopy